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Article

Detection and genotyping of SHV beta-lactamase variants by mass spectrometry after base-specific cleavage of in vitro-generated RNA transcripts.

Journal of clinical microbiology - 1 Mar 2006

Stürenburg Enno, Storm Niels, Sobottka Ingo, Horstkotte Matthias A, Scherpe Stefanie, Aepfelbacher Martin, Müller Susanne

Abstract excerpt

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) after base-specific cleavage of PCR-amplified and in vitro-transcribed bla(SHV) genes was used for the identification and genotyping of SHV beta-lactamases. For evaluation, bla(SHV) stretches of 21 clinical Enterobacteriaceae isolates were PCR amplified using T7 promoter-tagged forward and reverse primers, respectively. In...

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Identifiers

PMID: 16517875
DOI: 10.1128/JCM.44.3.909-915.2006
PMCID: PMC1393099

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Detection and genotyping of SHV beta-lactamase variants by mass spectrometry after base-specific cleavage of in vitro-generated RNA transcripts.PMID 16517875Read ↗

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Real-time PCR and melting curve analysis for reliable and rapid detection of SHV extended-spectrum beta-lactamases.Semantic proximity Read ↗

Investigation of genetic diversity of the bla(SHV) gene and development of an oligonucleotide microarray to detect mutations in the bla(SHV) gene.Semantic proximity Read ↗

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Application of minimal sequence quality values prevents misidentification of the blaSHV type in single bacterial isolates carrying different SHV extended-spectrum beta-lactamase genes.Semantic proximity Read ↗

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