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Article

Discrimination of SHV beta-lactamase genes by restriction site insertion-PCR.

Antimicrobial agents and chemotherapy - 1 Jul 2001

Chanawong A, M'Zali F H, Heritage J, Lulitanond A, Hawkey P M

Abstract excerpt

Restriction site insertion-PCR (RSI-PCR) is a simple, rapid technique for detection of point mutations. This technique exploits primers with one to three base mismatches near the 3' end to modulate a restriction site. We have developed this technique to identify described mutations of the bla(SHV) genes for differentiation of SHV variants that cannot be distinguished easily by other techniques. To validate this...

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Identifiers

PMID: 11408231
DOI: 10.1128/AAC.45.7.2110-2114.2001
PMCID: PMC90608

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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