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Article

Analysis of bla(SHV) codon 238 and 240 allele mixtures using Sybr green high-resolution melting analysis.

Antimicrobial agents and chemotherapy - 1 Jun 2009

Andersson Patiyan, Harris Tegan, Tong Steven Y C, Giffard Philip M

Abstract excerpt

Klebsiella pneumoniae isolates frequently contain complex mixtures of bla(SHV) alleles. A high-resolution melting-based method for interrogating the extended-spectrum activity conferring codon 238 and 240 polymorphisms was developed. This detects minority extended-spectrum beta-lactamase-encoding alleles, allows estimation of allele ratios, and discriminates between single and double mutants.

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Identifiers

PMID: 19349515
DOI: 10.1128/AAC.00063-09
PMCID: PMC2687234

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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