Article
Negative epistasis between natural variants of the Saccharomyces cerevisiae MLH1 and PMS1 genes results in a defect in mismatch repair.
Proceedings of the National Academy of Sciences of the United States of America - 28 Feb 2006
Heck Julie Akiko, Argueso Juan Lucas, Gemici Zekeriyya, Reeves Richard Guy, Bernard Ann, Aquadro Charles F, Alani Eric
Abstract excerpt
In budding yeast, the MLH1-PMS1 heterodimer is the major MutL homolog complex that acts to repair mismatches arising during DNA replication. Using a highly sensitive mutator assay, we observed that Saccharomyces cerevisiae strains bearing the S288c-strain-derived MLH1 gene and the SK1-strain-derived PMS1 gene displayed elevated mutation rates that conferred a long-term fitness cost. Dissection of this negative...
Topics
- Adaptor Proteins, Signal Transducing
- Base Pair Mismatch
- Carrier Proteins
- DNA Repair
- DNA, Fungal
- DNA, Recombinant
- Epistasis, Genetic
- Evolution, Molecular
- Fungal Proteins
- Molecular Sequence Data
- MutL Protein Homolog 1
- MutL Proteins