Identification of Transcriptionally Active Open Reading Frames within the RD1 Genomic Segment of <i>Mycobacterium tuberculosis</i>

Abstract excerpt

OBJECTIVE: To identify transcriptionally active open reading frames (ORFs), predicted by bioinformatics, within RD1 genomic segment of Mycobacterium tuberculosis using reverse transcription-polymerase chain reaction (RT-PCR). MATERIALS AND METHODS: M. tuberculosis H37Rv was grown in Middlebrook 7H9 medium for 8 weeks and total RNA was isolated using standard procedures. The cDNA was synthesized using first-strand...

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