A novel approach to assess insulin sensitivity reveals no increased insulin sensitivity in mice with a dominant-negative mutant hepatocyte nuclear factor-1alpha.

Abstract excerpt

In phenotype experiments in mice, determination of dynamic insulin sensitivity often uses the insulin tolerance test. However, the interpretation of this test is complicated by the counterregulation occurring at low glucose. To overcome this problem, we determined the dynamic insulin sensitivity after inhibition of endogenous insulin secretion by diazoxide (25 mg/kg) in association with intravenous administration...

Topics

• Animals
• Blood Glucose
• Diazoxide
• Female
• Gene Expression Regulation
• Genes, Dominant
• Glucose Clamp Technique
• Glucose Tolerance Test
• Hepatocyte Nuclear Factor 1-alpha
• Insulin
• Insulin Resistance
• Islets of Langerhans
• Mice
• Mice, Inbred C57BL
• Mutation