Intramolecular second-site revertants to the phosphorylation site mutation in OmpR, a kinase-dependent transcriptional activator in Escherichia coli.

doi:10.1128/jb.173.12.3749-3755.1991

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Article

Intramolecular second-site revertants to the phosphorylation site mutation in OmpR, a kinase-dependent transcriptional activator in Escherichia coli.

Journal of bacteriology - 1 Jun 1991

Brissette R E, Tsung K L, Inouye M

Abstract excerpt

OmpR is a transcriptional activator for the ompF and ompC genes of Escherichia coli. Its phosphorylation is mediated by a transmembrane sensory-receptor protein, EnvZ, and is essential for transcriptional activation. In a previous study, when the aspartic acid residue at position 55, the putative phosphorylation site, was replaced with glutamine (D55Q), ompF and ompC expression were completely lost. In this study...

Topics

Bacterial Outer Membrane Proteins
Base Sequence
DNA Fingerprinting
DNA, Bacterial
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Escherichia coli Proteins
Genes, Bacterial
Molecular Sequence Data
Multienzyme Complexes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Phosphoprotein Phosphatases

Phosphorylation

Plasmids

Protein Conformation

Trans-Activators

Source

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Identifiers

PMID: 1646788
DOI: 10.1128/jb.173.12.3749-3755.1991
PMCID: PMC208004

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1646788-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Intramolecular second-site revertants to the phosphorylation site mutation in OmpR, a kinase-dependent transcriptional activator in Escherichia coli.PMID 1646788Read ↗

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Signal transduction and osmoregulation in Escherichia coli. A novel type of mutation in the phosphorylation domain of the activator protein, OmpR, results in a defect in its phosphorylation-dependent DNA binding.Semantic proximity Read ↗

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Transmembrane signal transduction and osmoregulation in Escherichia coli: I. Analysis by site-directed mutagenesis of the amino acid residues involved in phosphotransfer between the two regulatory components, EnvZ and OmpR.Semantic proximity Read ↗

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