Transmembrane signal transduction and osmoregulation in Escherichia coli: I. Analysis by site-directed mutagenesis of the amino acid residues involved in phosphotransfer between the two regulatory components, EnvZ and OmpR.

Abstract excerpt

Previously, the transfer of a phosphoryl group between the EnvZ and OmpR proteins, which are involved in expression of the ompF and ompC genes in response to the medium osmolarity, was demonstrated in vitro. In this study, the histidine (His) residue at position 243 of the EnvZ protein, and the aspartate (Asp) residues at positions 12 and 55 of the OmpR protein were changed, respectively, by means of...

Topics

• Bacterial Outer Membrane Proteins
• Base Sequence
• Cloning, Molecular
• Escherichia coli
• Escherichia coli Proteins
• Genes, Bacterial
• Molecular Sequence Data
• Multienzyme Complexes
• Mutagenesis
• Phenotype