Article
Two transcriptionally active OmpR mutants that do not require phosphorylation by EnvZ in an Escherichia coli cell-free system.
Journal of bacteriology - 1 Oct 1992
Bowrin V, Brissette R, Inouye M
Abstract excerpt
D55Q-T83A and D55Q-G94S, two pseudorevertants of the D55Q mutant OmpR, an Escherichia coli transcriptional activator, were isolated previously by R. Brissette, K. Tsung, and M. Inouye (J. Bacteriol. 173:3749-3755, 1991). These pseudorevertant OmpR proteins were purified and examined for their function as transcriptional activators in a cell-free system with an ompF DNA fragment. These proteins were...
Topics
- Bacterial Outer Membrane Proteins
- Escherichia coli
- Escherichia coli Proteins
- Multienzyme Complexes
- Mutation
- Phosphoprotein Phosphatases
- Phosphorylation
- Transcription Factors
- Transcription, Genetic
