Article
Signal transduction and osmoregulation in Escherichia coli. A novel type of mutation in the phosphorylation domain of the activator protein, OmpR, results in a defect in its phosphorylation-dependent DNA binding.
The Journal of biological chemistry - 15 Jun 1991
Nakashima K, Kanamaru K, Aiba H, Mizuno T
Abstract excerpt
The transcriptional factors, OmpR and EnvZ, are crucially involved in the osmotic regulation of ompF and ompC expression in Escherichia coli. The DNA binding ability of the positive regulator, OmpR, is modulated through its phosphorylation and dephosphorylation mediated by EnvZ in response to the medium osmolarity. In this study, two examples of a novel type of mutant ompR allele, ompR96A and ompR115S, whose...
Topics
- Adenosine Triphosphate
- Bacterial Outer Membrane Proteins
- Cytidine Triphosphate
- DNA-Binding Proteins
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression
- Kinetics
- Multienzyme Complexes
- Phenotype