Stepwise cloning and genetic organization of the seemingly unclonable HgiCII restriction-modification system from Herpetosiphon giganteus strain Hpg9, using PCR technique.

Abstract excerpt

The genes, hgiCIIR and hgiCIIM, that encode the HgiCII restriction and modification (R-M) system from Herpetosiphon giganteus strain Hpg9, an AvaII isoschizomer recognizing the sequence, GGATCC, were cloned in Escherichia coli. Cloning the respective hgiCIIM gene was achieved via in vitro selection both from a Sau3AI- and an NheI-generated plasmid gene library using AvaII, a commercially available isoschizomer of...

Topics

• Amino Acid Sequence
• Base Sequence
• Cloning, Molecular
• DNA Restriction-Modification Enzymes
• DNA, Bacterial
• Deoxyribonucleases, Type II Site-Specific
• Gram-Negative Aerobic Bacteria
• Molecular Sequence Data
• Phenotype
• Polymerase Chain Reaction