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Scalable Generation of High-Titer Recombinant Adeno-Associated Virus Type 5 in Insect Cells

26 Jan 2006

Abstract excerpt

We established a method for production of recombinant adeno-associated virus type 5 (rAAV5) in insect cells by use of baculovirus expression vectors. One baculovirus harbors a transgene between the inverted terminal repeat sequences of type 5, and the second expresses Rep78 and Rep52. Interestingly, the replacement of type 5 Rep52 with type 1 Rep52 generated four times more rAAV5 particles. We replaced the...

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PMID: 16439543
DOI: 10.1128/jvi.80.4.1874-1885.2006

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