Article
Complex interactions of HIV-1 nucleocapsid protein with oligonucleotides.
Nucleic acids research - 1 Jan 2006
Fisher Robert J, Fivash Matthew J, Stephen Andrew G, Hagan Nathan A, Shenoy Shilpa R, Medaglia Maxine V, Smith Lindsey R, Worthy Karen M, Simpson John T, Shoemaker Robert, McNitt Karen L, Johnson Donald G, Hixson Catherine V, Gorelick Robert J, Fabris Daniele, Henderson Louis E, Rein Alan
Abstract excerpt
The HIV-1 nucleocapsid (NC) protein is a small, basic protein containing two retroviral zinc fingers. It is a highly active nucleic acid chaperone; because of this activity, it plays a crucial role in virus replication as a cofactor during reverse transcription, and is probably important in other steps of the replication cycle as well. We previously reported that NC binds with high-affinity to the repeating...
Topics
- Binding Sites
- Binding, Competitive
- Calorimetry
- Capsid Proteins
- Fluorescence
- Fluorescence Polarization
- Gene Products, gag
- Mutation
- Oligodeoxyribonucleotides