Article
Characterization of enteropathogenic Escherichia coli mutants that fail to disrupt host cell spreading and attachment to substratum.
Infection and immunity - 1 Feb 2006
Nadler Chen, Shifrin Yulia, Nov Shani, Kobi Simi, Rosenshine Ilan
Abstract excerpt
Upon infection of host cells, enteropathogenic Escherichia coli (EPEC) delivers a set of effector proteins into the host cell cytoplasm via the type III secretion system (TTSS). The effectors subvert various host cell functions. We found that EPEC interferes with the spreading and ultimately with the attachment of suspended fibroblasts or epithelial cells, and we isolated mini-Tn10kan insertion mutants that...
Topics
- Cell Adhesion
- Cell Line
- Escherichia coli
- Escherichia coli Proteins
- Fibroblasts
- Gene Expression Regulation, Bacterial
- HeLa Cells
- Humans
- Mutation
- Phosphoproteins
- Protein Kinases
- Signal Transduction
- Transcription Factors