Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Failure to genotype Human Cytomegalovirus by PCR-RFLP method due to sequence variation within the primer binding site.

Journal of virological methods - 1 Jun 2006

Sowmya P, Madhavan H N, Therese K L

Abstract excerpt

Polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) is one of the rapid methods for genotyping Human Cytomegalovirus (HCMV). When genotyping clinical samples by a sensitive nested PCR-based RFLP method for the glycoprotein B (gB) gene of HCMV, it was found that some of the clinical specimens did not give an amplification signal. Analysis of the prototype sequences of the different...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Binding Sites
Share to Cytomegalovirus
Share to Cytomegalovirus Infections

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Primers

Share to Polymorphism, Restriction Fragment Length

Share to Sensitivity and Specificity

Share to Species Specificity

Share to Viral Envelope Proteins

Source

Identifiers

PMID: 16417928
DOI: 10.1016/j.jviromet.2005.12.001

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Failure to genotype Human Cytomegalovirus by PCR-RFLP method due to sequence variation within the primer binding site.PMID 16417928Read ↗

Genotyping of human cytomegalovirus using non-radioactive single-strand conformation polymorphism (SSCP) analysis.Semantic proximity Read ↗

Comparative efficacy of PCR-based restriction fragment length polymorphism (RFLP) & multiplex PCR for glycoprotein B (gB) genotyping of human cytomegalovirus.Semantic proximity Read ↗

Real-time PCR diagnostics failure caused by nucleotide variability within exon 4 of the human cytomegalovirus major immediate-early gene.Semantic proximity Read ↗

Epidemiology of human cytomegalovirus strains through comparison of methodological approaches to explore gN variants.Semantic proximity Read ↗

[Problems of cytomegalovirus diagnostics by real-time polymerase chain reaction.].Semantic proximity Read ↗

Human cytomegalovirus glycoprotein B genotypes in renal transplant recipients.Semantic proximity Read ↗

The type-2 variant of human cytomegalovirus glycoprotein N (gN-2) is not the rarest in the Chinese population of Taiwan: influence of primer design.Semantic proximity Read ↗

Determination of human cytomegalovirus genetic diversity in different patient populations in Costa Rica.Semantic proximity Read ↗

Sequence diversity in the glycoprotein B gene complicates real-time PCR assays for detection and quantification of cytomegalovirus.Semantic proximity Read ↗

Human cytomegalovirus glycoprotein B genotypes in blood of AIDS patients: lack of association with either the viral DNA load in leukocytes or presence of retinitis.Semantic proximity Read ↗

Human cytomegalovirus strains associated with congenital and perinatal infectionsSemantic proximity Read ↗

A fifth human cytomegalovirus glycoprotein B genotype.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.