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Article

Sequence diversity in the glycoprotein B gene complicates real-time PCR assays for detection and quantification of cytomegalovirus.

Journal of clinical microbiology - 1 Oct 2005

Nye Melinda B, Leman Adam R, Meyer Michelle E, Menegus Marilyn A, Rothberg Paul G

Abstract excerpt

Real-time quantitative PCR systems (Q-PCR) for the rapid detection and quantification of microorganisms in clinical specimens employ oligodeoxyribonucleotide primers and probes for specificity, which makes them vulnerable to false negatives caused by sequence diversity in the template. Schaade et al. (J. Clin. Microbiol. 39:3809, 2001) reported a sequence variant (C630T) in the cytomegalovirus (CMV) glycoprotein...

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Identifiers

PMID: 16207949
DOI: 10.1128/JCM.43.10.4968-4971.2005
PMCID: PMC1248473

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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