Article
Comparative efficacy of PCR-based restriction fragment length polymorphism (RFLP) & multiplex PCR for glycoprotein B (gB) genotyping of human cytomegalovirus.
The Indian journal of medical research - 1 Aug 2007
Sowmya P, Dhanya V, Madhavan H N, Therese K L
Abstract excerpt
BACKGROUND & OBJECTIVE: Glycoprotein B (gB), involved in cell-to-cell transmission of human cytomegalovirus (HCMV), is a critical factor in tissue tropism and viral pathogenesis. The aim of the present study was to compare the efficiency of PCR-based RFLP and multiplex nested PCR for gB gene of HCMV to determine their genotype in clinical specimens from patients with HCMV. METHODS: The PCR based RFLP and the...
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