Article
H19-DMR allele-specific methylation analysis reveals epigenetic heterogeneity of CTCF binding site 6 but not of site 5 in head-and-neck carcinomas: a pilot case-control analysis.
International journal of molecular medicine - 1 Feb 2006
De Castro Valente Esteves Leda Isabel, De Karla Cervigne Nilva, Do Carmo Javaroni Afonso, Magrin José, Kowalski Luiz Paulo, Rainho Cláudia Aparecida, Rogatto Silvia Regina
Abstract excerpt
Aberrant methylation of seven potential binding sites of the CTCF factor in the differentially methylated region upstream of the H19 gene (H19-DMR) has been suggested as critical for the regulation of IGF2 and H19 imprinted genes. In this study, we analyzed the allele-specific methylation pattern of CTCF binding sites 5 and 6 using methylation-sensitive restriction enzyme PCR followed by RFLP analysis in matched...
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