Transformation and characterization of mutant human fibroblasts defective in peroxisome assembly.

doi:10.1016/0014-4827(92)90278-g

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Article

Transformation and characterization of mutant human fibroblasts defective in peroxisome assembly.

Experimental cell research - 1 Aug 1992

Okamoto H, Suzuki Y, Shimozawa N, Yajima S, Masuno M, Orii T

Abstract excerpt

Human skin fibroblasts deficient in peroxisome biogenesis were transformed by transfecting SV40 ori- DNA with the use of an electroporator, and the biochemical, immunocytochemical, and cytogenetic properties of the transformants were analyzed. Cells (1 x 10(6)) from a patient with Zellweger syndr...

Topics

Acyltransferases
Adrenoleukodystrophy
Cell Line, Transformed
Dihydroxyacetone Phosphate
Fatty Acids
Fibroblasts
Humans
Microbodies
Mutation
Transformation, Genetic
Zellweger Syndrome

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