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Article

Phenotypic and functional characteristics of dendritic cells derived from human peripheral blood monocytes.

Journal of Zhejiang University. Science. B - 1 Dec 2005

Tang Ling-ling, Zhang Zhe, Zheng Jie-sheng, Sheng Ji-fang, Liu Ke-zhou

Abstract excerpt

OBJECTIVE: This study is aimed at developing a simple and easy way to generate dendritic cells (DCs) from human peripheral blood monocytes (PBMCs) in vitro. METHODS: PBMCs were isolated directly from white blood cell rather than whole blood and purified by patching methods (collecting the attached cell and removing the suspension cell). DCs were then generated by culturing PBMCs for six days with 30 ng/ml...

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Identifiers

PMID: 16358375
DOI: 10.1631/jzus.2005.B1176
PMCID: PMC1390640

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