Article
Novel seizure phenotype and sleep disruptions in knock-in mice with hypersensitive alpha 4* nicotinic receptors.
The Journal of neuroscience : the official journal of the Society for Neuroscience - 7 Dec 2005
Fonck Carlos, Cohen Bruce N, Nashmi Raad, Whiteaker Paul, Wagenaar Daniel A, Rodrigues-Pinguet Nivalda, Deshpande Purnima, McKinney Sheri, Kwoh Steven, Munoz Jose, Labarca Cesar, Collins Allan C, Marks Michael J, Lester Henry A
Abstract excerpt
A leucine to alanine substitution (L9'A) was introduced in the M2 region of the mouse alpha4 neuronal nicotinic acetylcholine receptor (nAChR) subunit. Expressed in Xenopus oocytes, alpha4(L9'A)beta2 nAChRs were > or =30-fold more sensitive than wild type (WT) to both ACh and nicotine. We generated knock-in mice with the L9'A mutation and studied their cellular responses, seizure phenotype, and sleep-wake cycle....
Topics
- Animals
- Dose-Response Relationship, Drug
- Female
- Mice
- Mice, Transgenic
- Mutation
- Nicotinic Agonists
- Phenotype
- Protein Subunits
- Receptors, Nicotinic
- Seizures
- Sleep Wake Disorders