Article
Interaction of dimethyl- and monomethyloxyluciferin with recombinant wild-type and mutant firefly luciferases.
Biochemistry. Biokhimiia - 1 May 2006
Vlasova T N, Leontieva O V, Ugarova N N
Abstract excerpt
Dissociation constants (Ks) in the pH range 6.5-9.0 for complexes of luciferin, dimethyloxyluciferin (DMOL), and monomethylluciferin (MMOL) with recombinant wild-type and mutant (His433Tyr) luciferases from the Luciola mingrelica firefly were determined by fluorescent titration. The protonated effectors were bound by the wild-type and mutant luciferases better than the nonprotonated ones. The affinity of DMOL for...
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