Article
Pyrosequencing Bacillus anthracis.
Emerging infectious diseases - 1 Oct 2005
Wahab Tara, Hjalmarsson Sandra, Wollin Ralfh, Engstrand Lars
Abstract excerpt
Pyrosequencing technology is a sequencing method that screens DNA nucleotide incorporation in real time. A set of coupled enzymatic reactions, together with bioluminescence, detects incorporated nucleotides in the form of light pulses, which produces a profile of characteristic peaks in a pyrogram. We used this technology to identify the warfare agent Bacillus anthracis by sequencing 4 single nucleotide...
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