Article
Rapid genotyping of Bacillus anthracis strains by real-time polymerase chain reaction.
Annals of the New York Academy of Sciences - 1 Oct 2002
Patra Guy, Williams Leanne E, Qi Yuan, Rose Sharon, Redkar Rajendra, Delvecchio Vito G
Abstract excerpt
Rapid and accurate identification of Bacillus anthracis is critical for patient care as well as outbreak control. We have developed 3 separate PCR based assays using fluorescence resonance energy transfer (FRET) to detect the presence of pXO1, pXO2 plasmids and a chromosomal marker. A set of amplification primers and probes were used in each assay. The probes were ad jacently placed inside the primer sites and...
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