Identification and Analysis of Informative Single Nucleotide Polymorphisms in 16S rRNA Gene Sequences of the Bacillus cereus Group.

doi:10.1128/JCM.01267-16

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Article

Identification and Analysis of Informative Single Nucleotide Polymorphisms in 16S rRNA Gene Sequences of the Bacillus cereus Group.

Journal of clinical microbiology - 1 Nov 2016

Hakovirta Janetta R, Prezioso Samantha, Hodge David, Pillai Segaran P, Weigel Linda M

Abstract excerpt

Analysis of 16S rRNA genes is important for phylogenetic classification of known and novel bacterial genera and species and for detection of uncultivable bacteria. PCR amplification of 16S rRNA genes with universal primers produces a mixture of amplicons from all rRNA operons in the genome, and the sequence data generally yield a consensus sequence. Here we describe valuable data that are missing from consensus...

Topics

Bacillus
Computational Biology
Humans
Polymorphism, Single Nucleotide
RNA, Ribosomal, 16S

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Identifiers

PMID: 27582514
DOI: 10.1128/JCM.01267-16
PMCID: PMC5078553

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