Article
Effects of mutation position on frequency of marker rescue by homologous recombination.
Molecular and cellular biology - 1 Aug 1992
Jiang L, Connor A, Shulman M J
Abstract excerpt
Homologous recombination between transferred and chromosomal DNA can be used for mapping mutations by marker rescue, i.e., by identifying which segment of wild-type DNA can recombine with the mutant chromosomal gene and restore normal function. In order to define how much the fragments should overlap each other for reliable mapping, we have measured how the frequency of marker rescue is affected by the position...
Topics
- Animals
- Cell Line
- DNA
- Genetic Markers
- Genetic Techniques
- Hybridomas
- Mutation
- Recombination, Genetic
- Transfection