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Article

Genetic assay for multimerization of retroviral gag polyproteins.

Journal of virology - 1 Aug 1992

Luban J, Alin K B, Bossolt K L, Humaran T, Goff S P

Abstract excerpt

We have established a genetic assay for the multimerization of retroviral gag polyproteins. This assay is based on the GAL4 two-hybrid system for studying protein-protein interactions (S. Fields and O. Song, Nature (London) 340:245-246, 1989). In our initial experiments, we generated Saccharomyces cerevisiae plasmids that separately express the GAL4 DNA-binding and GAL4 activation domains fused to the human...

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Identifiers

PMID: 1629970
DOI: 10.1128/JVI.66.8.5157-5160.1992
PMCID: PMC241396

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