Article
Genetic assay for multimerization of retroviral gag polyproteins.
Journal of virology - 1 Aug 1992
Luban J, Alin K B, Bossolt K L, Humaran T, Goff S P
Abstract excerpt
We have established a genetic assay for the multimerization of retroviral gag polyproteins. This assay is based on the GAL4 two-hybrid system for studying protein-protein interactions (S. Fields and O. Song, Nature (London) 340:245-246, 1989). In our initial experiments, we generated Saccharomyce...
Topics
- Cloning, Molecular
- DNA-Binding Proteins
- Fungal Proteins
- Gene Products, gag
- Genes, gag
- Genetic Techniques
- HIV-1
- Moloney murine leukemia virus
- Phenotype
- Plasmids
- Recombinant Fusion Proteins
- Retroviridae
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Transcription Factors
- beta-Galactosidase