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Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture

17 Nov 2005

Abstract excerpt

The use of a transgenic line of rats that express enhanced GFP (EGFP) exclusively in the germ line has allowed a separation of feeder layers and contaminating testis somatic cells from germ cells and the identification of a set of spermatogonial stem cell marker transcripts. With these molecular markers as a guide, we have now devised culture conditions where rat spermatogonial stem cells renew and proliferate in...

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PMID: 16293688
DOI: 10.1073/pnas.0508780102

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