Article
Biochemical properties of recombinant human beta-glucuronidase synthesized in baby hamster kidney cells.
The Biochemical journal - 1 Aug 1994
Gehrmann M C, Opper M, Sedlacek H H, Bosslet K, Czech J
Abstract excerpt
The cDNA sequence encoding human beta-glucuronidase [Oshima, Kyle, Miller, Hoffmann, Powell, Grubb, Sly, Troplak, Guise and Gravel (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 685-689] was expressed in baby hamster kidney (BHK) cells. After purification from the culture supernatant in one step by use of immunoaffinity chromatography, the biochemical properties of the enzyme were examined. With a pH optimum of 4.0, a...
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