Article
Biochemical properties of recombinant human beta-glucuronidase synthesized in baby hamster kidney cells.
The Biochemical journal - 1 Aug 1994
Gehrmann M C, Opper M, Sedlacek H H, Bosslet K, Czech J
Abstract excerpt
The cDNA sequence encoding human beta-glucuronidase [Oshima, Kyle, Miller, Hoffmann, Powell, Grubb, Sly, Troplak, Guise and Gravel (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 685-689] was expressed in baby hamster kidney (BHK) cells. After purification from the culture supernatant in one step by use...
Topics
- Animals
- Base Sequence
- Biological Transport
- Cell Line
- Chromatography, Affinity
- Chromatography, Gel
- Cricetinae
- DNA, Complementary
- Disulfides
- Endopeptidases
- Enzyme Stability
- Gene Expression
- Glucuronidase
- Humans
- Hydrogen-Ion Concentration
- Kidney
- Macromolecular Substances
- Molecular Sequence Data