Article
Ribosomes containing mutants of L4 ribosomal protein from Thermus thermophilus display multiple defects in ribosomal functions and sensitivity against erythromycin.
RNA (New York, N.Y.) - 1 Nov 2005
Tsagkalia Aikaterini, Leontiadou Fotini, Xaplanteri Maria A, Papadopoulos Georgios, Kalpaxis Dimitrios L, Choli-Papadopoulou Theodora
Abstract excerpt
Protein L4 from Thermus thermophilus (TthL4) was heterologously overproduced in Escherichia coli cells. To study the implication of the extended loop of TthL4 in the exit-tunnel and peptidyltransferase functions, the highly conserved E56 was replaced by D or Q, while the semiconserved G55 was changed to E or S. Moreover, the sequence -G55E56- was inverted to -E55G56-. When we incorporated these mutants into E....
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