Article
Molecular analysis of rpoB gene mutations in rifampicin resistant Mycobacterium tuberculosis isolates by multiple allele specific polymerase chain reaction in Puducherry, South India.
Journal of infection and public health - 1 Jan 2000
Thirumurugan Ravibalan, Kathirvel Maruthai, Vallayyachari Kommoju, Surendar Kesavan, Samrot Antony V, Muthaiah Muthuraj
Abstract excerpt
BACKGROUND: rpoB gene mutations in Mycobacterium tuberculosis (MTB) make the bacteria resistant to rifampicin. Thus, these mutations are surrogate markers for multi-drug resistance (MDR). The objective of this study was to evaluate an allele-specific multiplex-polymerase chain reaction (MAS-PCR) assay to detect mutations at codons 516, 526 and 531 of the rpoB gene. METHODS: In total, 127 M. tuberculosis clinical...
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