Article
The amino terminus of ADP-ribosylation factor (ARF) is a critical determinant of ARF activities and is a potent and specific inhibitor of protein transport.
The Journal of biological chemistry - 25 Jun 1992
Kahn R A, Randazzo P, Serafini T, Weiss O, Rulka C, Clark J, Amherdt M, Roller P, Orci L, Rothman J E
Abstract excerpt
Deletion of the amino-terminal 17 residues from human ADP-ribosylation factor (ARF) resulted in a protein ([delta 1-17]mARF1p) devoid of ARF activity but which retained the ability to bind guanine nucleotides with high affinity. Unlike the wild type, the binding of guanine nucleotides to this deletion mutant was found to be independent of added phospholipids. A chimeric protein was produced, consisting of 10%...
Topics
- ADP-Ribosylation Factor 1
- ADP-Ribosylation Factors
- Amino Acid Sequence
- Biological Transport
- Circular Dichroism
- Cloning, Molecular
- GTP-Binding Proteins
- Golgi Apparatus
- Kinetics
- Microscopy, Electron