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Article

Irreversible misfolding of diacylglycerol kinase is independent of aggregation and occurs prior to trimerization and membrane association.

Biochemistry - 22 Aug 2006

Mi Dehui, Kim Hak Jun, Hadziselimovic Arina, Sanders Charles R

Abstract excerpt

Escherichia coli diacylglycerol kinase (DAGK) is a homotrimeric helical integral membrane protein in which a number of single-site mutations to cysteine are known to promote misfolding. Here, effects of other amino acid replacements have been explored using a folding assay based on the dilution of acidic urea/DAGK stock solutions into detergent/lipid mixed micelles. DAGK with an I110P or I110R mutation in the...

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Identifiers

PMID: 16906765
DOI: 10.1021/bi060887x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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