Article
A modified procedure for replica plating of mammalian cells allowing selection of clones based on gene expression.
BioTechniques - 1 Feb 1992
Hornsby P J, Yang L, Lala D S, Cheng C Y, Salmons B
Abstract excerpt
The polyester cloth replica-plating technique for selection of mammalian cell clones was modified by growing cells in colonies on a flexible polytetrafluoroethylene membrane and then transferring them completely to polyester cloth (27-microns mesh), from which a replica was made by allowing cells to transfer to a cloth of smaller pore size (17-microns mesh). Using this technique, two phenotype selection methods...
Topics
- Animals
- Cats
- Cattle
- Cells, Cultured
- Clone Cells
- Cloning, Molecular
- Gene Expression
- Humans
- Luciferases
- Mammals
- Nucleic Acid Hybridization
- Phenotype