Article
On the relationships of substrate orientation, hydrogen abstraction, and product stereochemistry in single and double dioxygenations by soybean lipoxygenase-1 and its Ala542Gly mutant.
The Journal of biological chemistry - 18 Nov 2005
Coffa Gianguido, Imber Ann N, Maguire Brendan C, Laxmikanthan Gurunathan, Schneider Claus, Gaffney Betty J, Brash Alan R
Abstract excerpt
Recent findings associate the control of stereochemistry in lipoxygenase (LOX) catalysis with a conserved active site alanine for S configuration hydroperoxide products, or a corresponding glycine for R stereoconfiguration. To further elucidate the mechanistic basis for this stereocontrol we compared the stereoselectivity of the initiating hydrogen abstraction in soybean LOX-1 and an Ala542Gly mutant that...
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