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Article

Investigation of substrate binding and product stereochemistry issues in two linoleate 9-lipoxygenases.

Lipids - 1 Nov 2008

Boeglin William E, Itoh Aya, Zheng Yuxiang, Coffa Gianguido, Howe Gregg A, Brash Alan R

Abstract excerpt

Herein we characterize the Arabidopsis thaliana AtLOX1 and tomato (Solanum lycopersicum) LOXA proteins as linoleate 9S-lipoxygenases (9-LOX), and use the enzymes to test a model that predicts a relationship between substrate binding orientation and product stereochemistry. The cDNAs were heterologously expressed in E. coli and the proteins partially purified by nickel affinity chromatography using a N-terminal...

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Identifiers

PMID: 18795358
DOI: 10.1007/s11745-008-3230-1
PMCID: PMC4710486

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