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Article

Detection of allelic variants of the canine IGHA gene by fluorescence resonance energy transfer melting temperature examination.

Journal of immunological methods - 1 Sept 2005

Peters I R, Helps C R, Lait P L, Harris C, Lee A C, Jones C A, Hall E J, Day M J

Abstract excerpt

The fluorescence resonance energy transfer (FRET) dual hybridisation probe system has been used for the detection of the accumulation of target DNA during real-time PCR and for the identification of nucleotide polymorphisms through examination of melt curves. This system involves the use of two oligonucleotide probes which are located close to each other and are complementary to an internal segment of a target...

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Identifiers

PMID: 16140319
DOI: 10.1016/j.jim.2005.06.009

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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