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Article

Novel antifolate resistant mutations of Plasmodium falciparum dihydrofolate reductase selected in Escherichia coli.

Molecular and biochemical parasitology - 1 Mar 2002

Chusacultanachai Sudsanguan, Thiensathit Pornpan, Tarnchompoo Bongkoch, Sirawaraporn Worachart, Yuthavong Yongyuth

Abstract excerpt

A simple and effective system has been developed from which a number of Plasmodium falciparum dihydrofolate reductase (pfDHFR) mutants conferring resistance to antifolates were randomly generated and characterized. The system exploited error-prone PCR to generate random mutations in the pfDHFR. Using the synthetic gene encoding for wild-type and quadruple mutant (N51I+C59R+S108N+I164L) pfDHFRs as templates,...

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Source

Identifiers

PMID: 11849706
DOI: 10.1016/s0166-6851(01)00440-6

Genes

DHFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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