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Article

Construction, detection and microarray analysis on the Shigella flexneri 2a sitC mutant.

Science in China. Series C, Life sciences - 1 Jun 2005

Liu Moqing, Liu Hongt, Sun Lilian, Dong Jie, Xue Ying, Chen Shuxia, Jin Qi

Abstract excerpt

In order to overcome the defects of difficult gene operations in low-copy suicide plasmid pCVD442, Gateway technology was applied in the construction process of recombinant plasmid for gene knockout in this study. With this improved knockout system, we inactivated sitC gene, which is associated with iron transport in Shigella flexneri 2a strain 301, to yield the mutant, MTS. The functional detection of the mutant...

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PMID: 16092755
DOI: 10.1007/BF03183616

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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