Article
Construction, detection and microarray analysis on Shigella dysenteriae a1 IroN, ShuA single, double mutants.
Science in China. Series C, Life sciences - 1 Jun 2006
Bin Wen, Liu Moqing, Peng Junping, Sun Lilian, Xu Xingye, Zhang Jinghai, Jin Qi
Abstract excerpt
In this study, we constructed single mutants MTS-1, MTS-2 of IroN and ShuA gene and double mutant MTS of them in Shigella dysenteriae A1 strain 51197 by insert and absence. The functional detection of every mutant was performed at the level of culture medium and cell experiment. The gene expression profiles of the mutants and the wild-type strains under iron-enriched and iron-limited conditions were analyzed by...
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