Article
Genetic analysis of human immunodeficiency virus type 1 nef in Portugal: subtyping, identification of mosaic genes, and amino acid sequence variability.
Journal of medical virology - 1 Sept 2005
Parreira Ricardo, Pádua Elizabeth, Piedade João, Venenno Teresa, Paixão Maria Teresa, Esteves Aida
Abstract excerpt
Extending our previous genetic characterization of human immunodeficiency virus type 1 (HIV-1) strains circulating in Portugal, we here report the first phylogenetic and putative amino acid sequence variability analyses of nef accessory gene. Viral sequences (n = 53) were amplified by nested PCR from proviral DNA purified from peripheral blood mononuclear cells of HIV-1 infected individuals (n = 49). Phylogenetic...
Topics
- Adolescent
- Adult
- Amino Acid Sequence
- Consensus Sequence
- DNA, Viral
- Female
- Genes, env
- Genes, nef
- Genetic Variation
- HIV Infections
- HIV-1
- Humans
- Male
- Middle Aged
- Phylogeny