Article
The comet assay approach to senescent human diploid fibroblasts identifies different phenotypes and clarifies relationships among nuclear size, DNA content, and DNA damage.
The journals of gerontology. Series A, Biological sciences and medical sciences - 1 Jun 2005
Mocali Alessandra, Giovannelli Lisa, Dolara Piero, Paoletti Francesco
Abstract excerpt
The comet assay methodology was used to monitor nuclear changes occurring in MRC5 human fibroblasts during transition from young to senescent cultures and to study heterogeneity of senescent populations. Nuclear morphology and size, DNA content per nucleus, and DNA damage (basal strand break, total damage, and oxidized base levels) were evaluated; moreover, visually identified large and small nuclei were analyzed...
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