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Quantitative DNA damage and repair measurement with the yeast comet assay.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012

Oliveira Rui, Johansson Björn

Abstract excerpt

The yeast comet assay is a fast, sensitive, and inexpensive technique to measure oxidative DNA damage, DNA damage repair, and the genotoxic or protective effects of chemicals. The main advantage over the comet assay using cells of higher organisms is the genetic tractability and ease of cultivation of yeast. A drawback is the lower DNA content of the cells as well as the need for cell wall digestion prior to...

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PMID: 22941599
DOI: 10.1007/978-1-61779-998-3_8

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