Article
Genetic approach to study the relationship between penicillin-binding protein 3 mutations and Haemophilus influenzae beta-lactam resistance by using site-directed mutagenesis and gene recombinants.
Antimicrobial agents and chemotherapy - 1 Jul 2005
Osaki Yumi, Sanbongi Yumiko, Ishikawa Midori, Kataoka Hiroshi, Suzuki Takahisa, Maeda Kumiko, Ida Takashi
Abstract excerpt
To clarify the relationship between mutations commonly found for penicillin-binding protein 3 (PBP 3) of beta-lactamase-nonproducing ampicillin-resistant (BLNAR) Haemophilus influenzae isolates and beta-lactam resistance, single and multiple amino acid mutations at positions 377, 385, 389, 517, and 526 were introduced into PBP 3 of a beta-lactam-susceptible Rd strain by site-directed mutagenesis. Twelve isogenic...
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