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Article

Creation of the first anomeric D/L-sugar kinase by means of directed evolution.

Proceedings of the National Academy of Sciences of the United States of America - 11 Nov 2003

Hoffmeister Dirk, Yang Jie, Liu Lesley, Thorson Jon S

Abstract excerpt

Chemoenzymatic routes toward complex glycoconjugates often depend on the availability of sugar-1-phosphates. Yet the chemical synthesis of these vital components is often tedious, whereas natural enzymes capable of anomeric phosphorylation are known to be specific for one or only a few monosaccharides. Herein we describe the application of directed evolution and a high-throughput multisugar colorimetric screen to...

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Identifiers

PMID: 14612558
DOI: 10.1073/pnas.100.23.13184
PMCID: PMC263743

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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