Article
Characterization of human primary enamel organ epithelial cells in vitro.
Archives of oral biology - 1 Aug 2005
DenBesten P K, Machule D, Zhang Y, Yan Q, Li W
Abstract excerpt
UNLABELLED: Tooth enamel is formed by ameloblasts, which are derived from the epithelial cells of the enamel organ. OBJECTIVE: The purpose of this study was to grow human ameloblast-like epithelial cells in culture. DESIGN: Human fetal tooth organs were isolated, and the cells were separated by digestion in collagenase/dispase. The cells were cultured in KGM-2 media with and without serum and at different calcium...
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