Article
Identification of the structural determinants for the stability of substrate and aminoacrylate external Schiff bases in O-acetylserine sulfhydrylase-A.
Biochemistry - 27 Jul 2010
Tian Hui, Guan Rong, Salsi Enea, Campanini Barbara, Bettati Stefano, Kumar Vidya Prasanna, Karsten William E, Mozzarelli Andrea, Cook Paul F
Abstract excerpt
O-Acetylserine sulfhydrylase is a pyridoxal 5'-phosphate (PLP)-dependent enzyme that catalyzes the final step in the cysteine biosynthetic pathway in enteric bacteria and plants, the replacement of the beta-acetoxy group of O-acetyl-L-serine (OAS) by a thiol to give L-cysteine. Previous studies of the K41A mutant enzyme showed L-methionine bound in an external Schiff base (ESB) linkage to PLP as the enzyme was...
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