Article
Role of Histidine-152 in cofactor orientation in the PLP-dependent O-acetylserine sulfhydrylase reaction.
Archives of biochemistry and biophysics - 15 Apr 2008
Tai Chia-Hui, Rabeh Wael M, Guan Rong, Schnackerz Klaus D, Cook Paul F
Abstract excerpt
O-Acetylserine sulfhydrylase catalyzes the final step of the biosynthesis of l-cysteine, the replacement of the beta-acetoxy group of O-acetyl-l-serine (OAS) by a thiol. The 5'-phosphate of the PLP cofactor is very tightly bound to the enzyme; it accepts 8 hydrogen bonds from enzyme side chains and a pair of water molecules, and is in close proximity to a helix dipole. Histidine-152 (H152) is one of the residues...
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