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Article

Probing heme protein-ligand interactions by UV/visible absorption spectroscopy.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2005

Nienhaus Karin, Nienhaus G Ulrich

Abstract excerpt

Ultraviolet/visible (UV/vis) absorption spectroscopy is a powerful tool for steady-state and time-resolved studies of protein-ligand interactions. Prosthetic groups in proteins frequently have strong electronic absorbance bands that depend on the oxidation, ligation, and conformation states of the chromophores. They are also sensitive to conformational changes of the polypeptide chain into which they are...

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Source

Identifiers

PMID: 15940000
DOI: 10.1385/1-59259-912-5:215

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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