Article
Association between Archaeal prolyl- and leucyl-tRNA synthetases enhances tRNA(Pro) aminoacylation.
The Journal of biological chemistry - 15 Jul 2005
Praetorius-Ibba Mette, Rogers Theresa E, Samson Rachel, Kelman Zvi, Ibba Michael
Abstract excerpt
Aminoacyl-tRNA synthetase-containing complexes have been identified in different eukaryotes, and their existence has also been suggested in some Archaea. To investigate interactions involving aminoacyl-tRNA synthetases in Archaea, we undertook a yeast two-hybrid screen for interactions between Methanothermobacter thermautotrophicus proteins using prolyl-tRNA synthetase (ProRS) as the bait. Interacting proteins...
Topics
- Amino Acyl-tRNA Synthetases
- Chromatography
- Chromatography, Gel
- DNA, Complementary
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gene Library
- Histidine
- Kinetics
- Leucine-tRNA Ligase
- Methanobacteriaceae
- Phenotype
- Protein Binding